ATP Binding Turns Plant Cryptochrome Into an Efficient Natural Photoswitch - Diatom Genomics Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2014

ATP Binding Turns Plant Cryptochrome Into an Efficient Natural Photoswitch

Résumé

Cryptochromes are flavoproteins that drive diverse developmental light-responses in plants and participate in the circadian clock in animals. Plant cryptochromes have found application as photoswitches in optogenetics. We have studied effects of pH and ATP on the functionally relevant photoreduction of the oxidized FAD cofactor to the semi-reduced FADH ? radical in isolated Arabidopsis cryptochrome 1 by transient absorption spectroscopy on nanosecond to millisecond timescales. In the absence of ATP, the yield of light-induced radicals strongly decreased with increasing pH from 6.5 to 8.5. With ATP present, these yields were significantly higher and virtually pH-independent up to pH 9. Analysis of our data in light of the crystallographic structure suggests that ATP-binding shifts the pK a of aspartic acid D396, the putative proton donor to FAD? 2 , from ,7.4 to .9, and favours a reaction pathway yielding long-lived aspartate D396 2. Its negative charge could trigger conformational changes necessary for signal transduction.
Fichier principal
Vignette du fichier
srep05175.pdf (1.59 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01358996 , version 1 (01-09-2016)

Licence

Paternité

Identifiants

Citer

Pavel Müller, Jean-Pierre Bouly, Kenichi Hitomi, Véronique Balland, Elizabeth D. Getzoff, et al.. ATP Binding Turns Plant Cryptochrome Into an Efficient Natural Photoswitch. Scientific Reports, 2014, 4, pp.5175. ⟨10.1038/srep05175⟩. ⟨hal-01358996⟩
258 Consultations
207 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More