Multi-scale mechanical characterization of prostate cancer cell lines : relevant biological markers to evaluate the cell metastatic potential - Archive ouverte HAL Access content directly
Journal Articles Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Year : 2017

Multi-scale mechanical characterization of prostate cancer cell lines : relevant biological markers to evaluate the cell metastatic potential

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Abstract

BackgroundConsidering the importance of cellular mechanics in the birth and evolution of cancer towards increasingly aggressive stages, we compared nano-mechanical properties of non-tumoral (WPMY-1) and highly aggressive metastatic (PC-3) prostate cell lines both on cell aggregates, single cells, and membrane lipids.MethodsCell aggregate rheological properties were analyzed during dynamic compression stress performed on a homemade rheometer. Single cell visco-elasticity measurements were performed by Atomic Force Microscopy using a cantilever with round tip on surface-attached cells. At a molecular level, the lateral diffusion coefficient of total extracted lipids deposited as a Langmuir monolayer on an air-water interface was measured by the FRAP technique.ResultsAt cellular pellet scale, and at single cell scale, PC-3 cells were less stiff, less viscous, and thus more prone to deformation than the WPMY-1 control. Interestingly, stress-relaxation curves indicated a two-step response, which we attributed to a differential response coming from two cell elements, successively stressed. Both responses are faster for PC-3 cells. At a molecular scale, the dynamics of the PC-3 lipid extracts are also faster than that of WPMY-1 lipid extracts.ConclusionsAs the evolution of cancer towards increasingly aggressive stages is accompanied by alterations both in membrane composition and in cytoskeleton dynamical properties, we attribute differences in viscoelasticity between PC-3 and WPMY-1 cells to modifications of both elements.General significanceA decrease in stiffness and a less viscous behavior may be one of the diverse mechanisms that cancer cells adopt to cope with the various physiological conditions that they encounter.
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Dates and versions

hal-01739046 , version 1 (20-03-2018)

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J. Zouaoui, A. Trunfio-Sfarghiu, L. Brizuela, Agnès Piednoir, O. Maniti, et al.. Multi-scale mechanical characterization of prostate cancer cell lines : relevant biological markers to evaluate the cell metastatic potential. Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects, 2017, 1861 (12), pp.3109 - 3119. ⟨10.1016/j.bbagen.2017.09.003⟩. ⟨hal-01739046⟩
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