Glass Microsphere-Supported Giant Vesicles for the Observation of Self-Reproduction of Lipid Boundaries

Michele Fiore 1 Ofelia Maniti 2 Agnes Girard-Egrot 2 Pierre-Alain Monnard 3 Peter Strazewski 1
1 CO2GLYCO - Chimie Organique 2-Glycochimie
ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
2 GEMBAS - Génie Enzymatique, Membrane Biomimétique et Assemblages Supramoléculaires
ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
3 Institute of Physics, Chemistry and Pharmacy,
Abstract : Growth and division experiments on phospholipid boundaries were carried out using glass microsphere‐supported phospholipid (DOPC) giant vesicles (GVs) fed with a fatty acid solution (oleic acid) at two distinct feeding rates. Both fast and slow feeding methods produced daughter GVs. Under slow feeding conditions the membrane growth process (evagination, buds, filaments) was observed in detail by fluorescence microscopy. The density difference between supported mother vesicles and newly formed daughter vesicles allowed their easy separation. Mass spectrometric analysis of the resulting mother and daughter GVs showed that the composition of both vesicle types was a mixture of original supported phospholipids and added fatty acids reflecting the total composition of amphiphiles after the feeding process. Thus, self‐reproduction of phospholipid vesicles can take place under preservation of the lipid composition but different aggregate size.
Keywords : Epifluoreszenzmikroskopie Immobilisierte Riesenvesikel Lipidgrenzflächen Wachstum und Teilung
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Journal articles
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https://hal-udl.archives-ouvertes.fr/hal-02116118
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Submitted on : Tuesday, April 30, 2019 - 5:02:25 PM
Last modification on : Wednesday, May 22, 2019 - 4:24:07 PM

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UNIV-LYON1 | INC-CNRS | INSA-LYON | INSA-GROUPE

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Michele Fiore, Ofelia Maniti, Agnes Girard-Egrot, Pierre-Alain Monnard, Peter Strazewski. Glass Microsphere-Supported Giant Vesicles for the Observation of Self-Reproduction of Lipid Boundaries. Angewandte Chemie, Wiley-VCH Verlag, 2018, 130 (1), pp.288-292. ⟨10.1002/ange.201710708⟩. ⟨hal-02116118⟩

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