Skip to Main content Skip to Navigation
Journal articles

Surfaces based on amino acid functionalized polyelectrolyte films towards active surfaces for enzyme immobilization

Abstract : Surface based on polyelectrolytes functionalized with amino acids onto amino-terminated solid surfaces of silicon wafers was prepared, with the purpose of evaluate the chemical functionality of the polyelectrolyte films in adsorption and catalytic activity of an enzyme. In this work, the adsorption of the enzyme glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides (LmG6PD) was studied as model. The polyelectrolytes were obtained from poly (maleic anhydride-alt-vinylpyrrolidone) [poly(MA-alt-VP)] and functionalized with amino acids of different hydropathy index: glutamine (Gln), tyrosine (Tyr) and methionine (Met). The polyelectrolytes were adsorbed onto the amino-terminated silicon wafer at pH 3.5 and 4.5 and at low and high ionic strength. At low ionic strength and pH 3.5, the largest quantity of adsorbed polyelectrolyte was on the films containing glutamine moiety as the most hydrophilic amino acid in the side chain of polymer chain (5.88 mg/m 2), whereas at high ionic strength and pH 4.5, the lowest quantity was in films containing tyrosine moiety in the side chain (1.88 mg/m 2). The films were characterized by ellipsometry, contact angle measurements and atomic force microscopy (AFM). The polyelectrolyte films showed a moderate degree of hydrophobicity, the methionine derivative being the most hydrophobic film. With the aim of evaluate the effect of the amino acid moieties on the ability of the surface to adsorb enzymes, we study the activity of the enzyme on these surfaces. We observed that the polarity of the side chain of the amino acid in the polyelectrolyte affected the quantity of LmG6PD adsorbed, as well as its specific activity, showing that films prepared from poly(MA-alt-VP) functionalized with Met provide the best enzymatic performance. The results obtained demonstrated that the surfaces prepared from polyelectrolytes functionalized with amino acids could be an attractive and simple platform for the im-mobilization of enzymes, which could be of interest for biocatalysis applications.
Document type :
Journal articles
Complete list of metadatas

Cited literature [78 references]  Display  Hide  Download

https://hal-udl.archives-ouvertes.fr/hal-02193218
Contributor : Umr5246 Icbms <>
Submitted on : Wednesday, July 8, 2020 - 11:40:38 AM
Last modification on : Thursday, July 16, 2020 - 3:16:31 AM

File

Amino Acid Functionalized.pdf
Files produced by the author(s)

Identifiers

Citation

Ximena Briones, Valeria Villalobos, Yves Queneau, Caroline Silva Danna, Rodrigo Muñoz, et al.. Surfaces based on amino acid functionalized polyelectrolyte films towards active surfaces for enzyme immobilization. Materials Science and Engineering: C, Elsevier, 2019, 104, pp.109938. ⟨10.1016/j.msec.2019.109938⟩. ⟨hal-02193218⟩

Share

Metrics

Record views

73

Files downloads

48