Gram-type differentiation of bacteria with 2D hollow photonic crystal cavities

R. Therisod (1) , M. Tardif (2, 3) , P. R. Marcoux (4) , E. Picard (5) , J.-B. Jager (5, 3, 6, 7) , E. Hadji (5) , D. Peyrade (2) , R. Houdré (1)

1 EPFL - Ecole Polytechnique Fédérale de Lausanne
2 LTM - Laboratoire des technologies de la microélectronique
3 UGA [2016-2019] - Université Grenoble Alpes [2016-2019]
4 DACLE-LETI - Département d'Architectures, Conception et Logiciels Embarqués-LETI
5 SiNaps - Silicon Nanoelectronics Photonics and Structures
6 PHELIQS - PHotonique, ELectronique et Ingénierie QuantiqueS
7 INAC - Institut Nanosciences et Cryogénie

Fast and label-free techniques to analyze viruses and bacteria are of crucial interest in biological and bio-medical applications. For this purpose, optofluidic systems based on the integration of photonic structures with microfluidic layers were shown to be promising tools for biological analysis, thanks to their small footprint and to their ability to manipulate objects using low powers. In this letter, we report on the optical trapping of living bacteria in a 2D silicon hollow photonic crystal cavity. This structure allows for the Gram-type differentiation of bacteria at the single cell scale, in a fast, label-free, and non-destructive way.

Domains: Biotechnology; Optics / Photonic; Micro and nanotechnologies/Microelectronics

R. Therisod, M. Tardif, P. R. Marcoux, E. Picard, J.-B. Jager, et al.. Gram-type differentiation of bacteria with 2D hollow photonic crystal cavities. Applied Physics Letters, 2018, 113 (11), pp.111101. ⟨10.1063/1.5037849⟩. ⟨cea-01988778⟩