High-throughput functional profiling of the human fungal pathogen Candida albicans genome

Murielle Chauvel (1) , Sophie Bachellier-Bassi (1) , Anne-Marie Guérout (2) , Keunsook K Lee (3, 4) , Corinne Maufrais (1, 5) , Emmanuelle Permal (1, 2, 5) , Juliana Pipoli da Fonseca (6) , Sadri Znaidi (1, 7) , Didier Mazel (2) , Carol A Munro (3) , Christophe D’enfert (1) , Melanie Legrand (1)

1 BPF - Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity
2 PGB - Plasticité du Génome Bactérien - Bacterial Genome Plasticity
3 Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
4 NGeneBio [Seoul, Corée du sud]
5 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
6 Biomics (plateforme technologique)
7 Institut Pasteur de Tunis

Candida albicans is a major fungal pathogen of humans. Although its genome has been sequenced more than two decades ago, there are still over 4300 uncharacterized C. albicans genes. We previously generated an ORFeome as well as a collection of destination vectors to facilitate overexpression of C. albicans ORFs. Here, we report the construction of ~2500 overexpression mutants and their evaluation by in vitro spotting on rich medium and in a liquid pool experiment in rich medium, allowing the identification of genes whose overexpression has a fitness cost. The candidates were further validated at the individual strain level. This new resource allows large-scale screens in different growth conditions to be performed routinely. Altogether, based on the concept of identifying functionally related genes by cluster analysis, the availability of this overexpression mutant collection will facilitate the character- ization of gene functions in C. albicans.

Keywords: Candida albicans; ORFeome; overexpression mutants; Bar-seq; Large-scale systematic studies; Candida albicans ORFeome Overexpression mutants Bar-seq Large-scale systematic studies

Domains: Life Sciences [q-bio]; Mycology

Murielle Chauvel, Sophie Bachellier-Bassi, Anne-Marie Guérout, Keunsook K Lee, Corinne Maufrais, et al.. High-throughput functional profiling of the human fungal pathogen Candida albicans genome. Research in Microbiology, 2023, 174 (3), pp.104025. ⟨10.1016/j.resmic.2022.104025⟩. ⟨pasteur-04131602⟩