A silicon micro-system for parallel gene transfection into arrayed cells

A. Tixier-Mita , J. Jun , M. Chiral , S. Ostrovidov , Marie Frénéa-Robin (1) , Bruno Le Pioufle (2) , H. Fujita

1 AMPERE - Ampère
2 SATIE - Systèmes et Applications des Technologies de l'Information et de l'Energie

We successfully made a silicon bio-micro-system able to capture several hundreds of cells as a regular array and to place each cell between microelectrodes for electroporation. We confirmed that some fluorescent dye was successfully inserted by electroporation in 75% of living captured cells; this is the first step towards parallel processing of gene insertion to individual cells.

Keywords: electroporation; gene transfection; bio-MEMS; arrayed cells

Domains: Electric power

A. Tixier-Mita, J. Jun, M. Chiral, S. Ostrovidov, Marie Frénéa-Robin, et al.. A silicon micro-system for parallel gene transfection into arrayed cells. 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Sep 2004, Malmo, Sweden. pp.180-182. ⟨hal-00412979⟩